Journal: The journal of pain

Article Title: A Critical Role for α7 Nicotinic Acetylcholine Receptor Signaling in Myeloid Lineage Cells in Chronic Neuropathic and Inflammatory Pain Responses

doi: 10.1016/j.jpain.2026.106339

Figure Lengend Snippet: Male and female adult C57BL/6J mice were treated with 8mg/kg paclitaxel (4 injections every other day) or vehicle according to the timeline. DRG and spinal cord tissues were dissected on days 7, 14, and 28 after the first dose, and qRT-PCR was performed. Values are expressed as mean ± SEM. n= 5/sex/group; *p<0.05 vs vehicle.

Article Snippet: Membranes were blocked with 5% milk before applying primary antibodies: α7 nAChR (Alomone ANC-007) and GAPDH (Cell Signaling 5174) at 4°C for 2 h. Horseradish peroxidase-conjugated secondary antibodies were obtained from Jackson Immunoresearch (West Grove, PA, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: The journal of pain

Article Title: A Critical Role for α7 Nicotinic Acetylcholine Receptor Signaling in Myeloid Lineage Cells in Chronic Neuropathic and Inflammatory Pain Responses

doi: 10.1016/j.jpain.2026.106339

Figure Lengend Snippet: C57BL/6J mice were treated with CFA or vehicle (n=4/sex/group). On day 3, DRG tissue was dissected, and qRT-PCR was performed. Values are expressed as normalized Ct. Results were analyzed using an unpaired t-test. **** p < 0.0001, vs. corresponding vehicle.

Article Snippet: Membranes were blocked with 5% milk before applying primary antibodies: α7 nAChR (Alomone ANC-007) and GAPDH (Cell Signaling 5174) at 4°C for 2 h. Horseradish peroxidase-conjugated secondary antibodies were obtained from Jackson Immunoresearch (West Grove, PA, USA).

Techniques: Expressing, Tissue, Quantitative RT-PCR

Journal: PLOS One

Article Title: NOX2 inhibition attenuates oxidative stress and eNOS uncoupling in pulmonary arteries of rats following simulated air diving

doi: 10.1371/journal.pone.0351145

Figure Lengend Snippet: (A) Concentration-response curves for endothelium-dependent relaxation to acetylcholine (ACh, 10 ⁻ ⁹–10 ⁻ ⁴ M) in isolated pulmonary artery rings from Group C, Group D, and Group D + GSK2795039. (B) Maximal relaxation (Emax) to ACh. (C) Concentration-response curves for endothelium-independent relaxation to sodium nitroprusside (SNP, 10 ⁻ ⁹–10 ⁻ ⁴ M) in isolated pulmonary artery rings from Group C, Group D, and Group D + GSK2795039. (D) Maximal relaxation (Emax) to SNP. Relaxation is expressed as the percentage of phenylephrine (PE, 10 μM)-induced pre-contraction. Data are presented as mean ± SD (n = 3 per group). Statistical analysis was performed using one-way ANOVA (for Emax) or two-way repeated-measures ANOVA (for curves) followed by Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Subsequently, endothelium-dependent relaxation was induced by cumulative addition of acetylcholine (ACh, 10 − 9 to 10 − 4 M; MedChem Express, NJ, USA; Cat. No. HY-B0282).

Techniques: Concentration Assay, Isolation